Location
Our discussion will be in the Nautilus meeting room, not the Chapel auditorium as initially announced. See the map below for directions.
Goals
The goal of the Technology working group for the week at Asilomar is to foster a robust discussion of the technologies and instruments we use to study extracellular vesicles.
- We plan to post the working group’s consensus about pros and cons of each technology here at asemv.org.
- We will also put together a white paper on the “EV Core” a research institution that wants to make an impact in the field should assemble. The document may start out as a very rough draft but will evolve as technologies mature. We welcome anyone interested in helping to keep the website and white paper up to date to join the working group.
- After discussions throughout the week, we will have a review session open to all new working group members on Thursday evening. The core WG members listed below will distill the week’s discussions into a first draft which we will distribute to the entire WG. Through email exchanges, online polls, and conference calls and in-person meetings as necessary, we will release the white paper and website recommendations after the working group reaches consensus.
ASEMV Technology Working Group core members
- Kendall Van Keuren-Jensen, TGen
- Louise Laurent, UC San Diego
- Michael Graner, UC Denver
- Heather O’Neill, Caris Life Sciences
- Julie Saugstad, Oregon Health Sciences University
- Roger Alexander, Extracellular RNA Communication Consortium
Agenda
9:30 – 9:55am Coffee and Registration
9:55 – 10:00am Opening Remarks, Kendall Van Keuren-Jensen
Flow Cytometry
10:00 – 10:45am
10:00 – 10:10am Rigorous and reproducible EV measurements by flow cytometry
John Nolan, Scintillon Institute
10:10 – 10:20am Know your limits
Jennifer Jones, NIH Pathology
10:20 – 10:30am A multi-dimensional approach to single-EV analysis by flow cytometry
Andries Zijlstra, Vanderbilt
10:30 – 10:45am Discussion
Microscopy
10:45 – 11:30am
10:45 – 10:55am Imaging of extracellular vesicles in living cells
Emanuele Cocucci, Ohio State University
10:55 – 11:05am Single molecule localization microscopy imaging of extracellular vesicles
Tijana Jovanovic-Talisman, City of Hope
11:05 – 11:15am Mapping sub-populations of extracellular vesicles by multimodal scanning probe imaging
Shivani Sharma, UCLA
11:15 – 11:30am Discussion
Proteomics
11:30 – 12:05pm
11:30 – 11:40am Aptamer-Based Affinity Labeling for target identification
Günter Mayer, University of Bonn
11:40 – 11:50am Quantitative proteomic techniques in exosome research
Pranav Sharma, Scripps Research
11:50 – 12:05pm Discussion
12:05 – 12:45pm Break (Box lunch provided)
Particle Characterization
12:45 – 1:30pm
12:45 – 12:55pm Isolation and characterization of heterogeneous populations of cancer-derived extracellular vesicles
Dolores Di Vizio, Cedars-Sinai Medical Center
12:55 – 1:05pm Tunable Resistive Pulse Sensing (TRPS) assessment of particle size and concentration
Ursula Sandau, Oregon Health Sciences University
1:05 – 1:15pm Technologies to unravel particle heterogeneity at single vesicle resolution
Randy Carney, UC Davis
1:15 – 1:30pm Discussion
Capture Technologies
1:30 – 2:15pm
1:30 – 1:40pm Large-scale purification of labeled exosomes by Tangential Flow Filtration
Dirk Dittmer, UNC Chapel Hill
1:40 – 1:50pm High resolution biophysical isolation, concentration, and characterization of complex bioparticles
Mark Hayes, Arizona State University
1:50 – 2:00pm Pros and cons of Cushioned-Density Gradient Ultracentrifugation for vesicle isolation
Robert Raffai, UC San Francisco
2:00 – 2:15pm Discussion
2:15 – 2:45pm Group Discussion
