Taught by leaders in the field, this 4-day class provides students with an exciting, up-to-date introduction to the field of exosomes, microvesicles, and other extracellular vesicles (EVs). Lectures cover our current understanding of the biochemistry, molecular, and cell biology of EV biogenesis and uptake, the functions and analysis of EV RNAs, and the latest approaches to purifying and analyzing exosomes and other EVs. These lectures are interwoven with hands-on laboratory instruction in which participants use well-validated protocols, reagents and kits from major manufacturers to purify exosomes and interrogate their protein and RNA content. Participants will leave the class with the conceptual framework and practical know-how necessary to explore the contribution of exosomes and other EVs to whatever biological question they are investigating.
Fatah Kashanchi (George Mason University) and Stephen J. Gould (Johns Hopkins University)