graphic: ASEMV 2014 at the Asilomar Conference Grounds

October 10–13, 2014
Asilomar Conference Grounds
San Francisco, CA

October 10, 2014
Session I
Chair: Douglas Taylor
Secretary-General, ASEMV

Xandra Breakefield, MGH/Harvard University
Extracellular RNA – Many forms and purposes

Gavin Wright, Wellcome Trust Sanger Institute, UK
Caris Discovery Lecture: Sperm meets egg: A role for vesicles in the membrane block to polyspermy?

Rama Khokha, Princess Margaret Cancer Centre, Canada
Timp function in harnessing the CAF cell state

Douglas Taylor, Exosome Sciences
Where’s Waldo? Vesicle isolation for research and clinical applications

Welcome Reception

October 11, 2014
Session II
Chair: Dolores DiVizio, Cedars-Sinai Medical Center

Stefan Momma, Frankfurt University, Germany
The vesicle behind the fusion behind the transdifferentiation – functional RNA transfer from blood to brain by extracellular vesicles.

Pamela Wearsch, Case Western Reserve University
Bacterial membrane vesicles mediate the secretion of immunomodulatory factors during Myocbacterium tuberculosis infection

David Wong, UC Los Angeles
The Landscape of Human Salivary Extracellular Non-Coding RNA

Alissa Weaver, Vanderbilt University
Regulation of cancer cell invasive behavior by secreted exosomes

Robert Raffai, UC San Francisco
Systemic Delivery of microRNA-146a Mimics in Lipid Microparticles Substitutes for ApoE in Suppressing Inflammation and Atherosclerosis in Hyperlipidemic Mice

Esbjörn Telemo, Gothenburg University, Sweden
Thymic exosomes promote the maturation of developing thymocytes

Makiko Ono, NCCRI, Tokyo
Exosomes from bone marrow mesenchymal stem cells contain a microRNA that promotes dormancy in metastatic breast cancer cells.

Andrew Kim, UC San Francisco
TMEM16F lipid scramblase is required for microvesicle formation in hematopoietic cells.

Jasmina Redzic, University of Colorado
Propagation of the unfolded protein response via extracellular vesicles in brain tumor cells

Matthew J. Shurtleff, UC Berkeley
Cell-Free Packaging of MicroRNA into Exosomes Reveals Y-box Protein I as a Critical Sorting Factor

Clark Chen, UC San Diego
miRNA contents of CSF extracellular vesicles (EVs) derived from glioblastoma patients

Session III
Chair: Michael Graner, University of Colorado

Prashanth Vallabhajosyula, U. of Pennsylvania
Characterization of transplant organ specific microvesicles

Michael Kriss, University of Colorado
Human liver sinusoidal endothelial cell-derived exosomes augment intrinsic anti-viral pathways in HCV-infected hepatocytes: Differential Effects of Type I vs Type III Interferons

Petra Hirsova, Mayo Clinic
Toxic lipids induce release of proinflammatory extracellular vesicles from hepatocytes

Ken Witwer, Johns Hopkins University
Effects of macrophage-derived EV and microRNAs on HIV infection and replication

Paula Saa, American Red Cross
Infectious prion proteins co-localize with plasma extracellular vesicles

Panisadee Avirutnan, Mahidol University, Thailand
A novel role for microparticles in dengue pathogenesis

Asuka Nanbo, Hokkaido University
Exosomes derived from Epstein-Barr Virus-infected cells are internalized via Caveolae- dependent endocytosis and promote phenotypic modulation in the target cells

Sarah Fernando, U. of British Columbia, Canada
Extracellular Vesicles Are Implicated in the Propagation of Misfolded Superoxide Dismutase in Amyotrophic Lateral Sclerosis Tissue

Kathleen Goodmon, Boston University
Neisseria gonorrhoeae induces production of exosomes in human epithelial cells, alters their content, and induces immune responses

Erez Eitan, NIA/NIH
Exosomes Containing Amyloid beta-Peptide and p-Tau as Predictive Biomarkers and Pathogenic Agents in Alzheimer’s Disease

Audrey Hubert, McGill University, Canada
Purification and characterisation of Extracellular Vesicles from plasma of HIV-1 patients

Amy Phillips, Izon, New Zealand
Recent Technical Developments in the Standardized Separation and Measurement of EVs and in the Detection and Measurement of Surface Proteins Using Aptamers

Open/Poster Viewing

Session IV
Chair: Xandra Breakefield, MGH/Harvard University

Fatah Kashanchi, George Mason University
Comparison of Exosomes from RNA viruses including HIV-1, HTLV-1 and Rift Valley Fever infected cells

Louise Laurent, UC San Diego
ExRNA isolation and analysis: current perspectives and goals for the future

Florin Selaru, Johns Hopkins University
Extracellular vesicle-mediated transfer of microRNAs in vivo for the treatment of cancer

Louise Laurent, UC San Diego
Gene Ontology for the exRNA field

Poster Session A – Food and open bar

October 12, 2014
Session V
Chair: Rama Khokha, Ontario Cancer Institute, Canada

Eduardo Marbán, Cedars-Sinai Medical Center
Disease-modifying activity of heart-derived exosomes opens up a new paradigm for therapeutics

Stephen Gould, Johns Hopkins University
Biogenesis of exosomal/microvesicular proteins

Saumya Das, BIDMC/Harvard University
A functional role for cardiomyocyte derived extracellular vesicles in cardiac remodeling

Horst Vogel, EPFL, Lausanne, Switzerland
A generic approach for isolating and characterizing extracellular vesicles

Elvira Donnarumma, IRCCS-SDN, Naples, Italy
Role of exosomal microRNAs of cancer-associated fibroblasts in breast cancer

Anush Arakelyn, NICHD/NIH
Flow-cytometric characterization of the antigenic spectra of individual extracellular vesicles

Masamitsu Kanada, Stanford University
Differential fates of biomolecules delivered to target cells via extracellular vesicles

Julie Saugstad, Oregon Health & Science University
MicroRNAs in human cerebrospinal fluid as biomarkers for Alzheimers disease

Sudipto Chakrabortty, Cold Spring Harbor Laboratory
Intercellular transfer of functional RNA molecules through exosomes

Sasha Vlassov, LifeTechnologies
Exploring the paths of exosome formation and secretion with next generation siRNA tools

Session VI
Chair: Leonora Balaj, MGH/Harvard University

Janos Zempleni, University of Nebraska
Bovine MicroRNAs are Bioavailable and Affect Gene Expression in Humans and Mice

Antonio de Maio, UCSD
Cellular communication via extracellular vesicles during stress

Michael Olin, University of Minnesota
Evaluating glioma-derived exosomes as the potential source of tumor-induced immunosuppression

Anne Knowlton, UC Davis
ROS, Exosomes and Adult Cardiac Myocytes

Travis Antes, SystemBiosciences
Programming Exosome Cargo and Targeting Cell Delivery

Daiki Yoshii, Kumamoto University, Japan
Exosomes and microvesicles mRNAs in human bile for the assessment of transplanted liver in the early postoperative period

Aurélie Cordouan, Laval University, Canada
Platelet microparticles and derived microRNAs reprogram primary human macrophage gene expression and function

Mikhail Skliar, University of Utah
Exosomes are surface active nanoparticles that self-assemble at interfaces: Evidence and applications

Asim Abdel-Mageed, Tulane University
Prostate Cancer Cell-Derived Exosomes in Oncogenic Reprogramming of Stem Cells

Vanille Greiner, UC San Francisco
A positive microRNA sensor circuit for in vivo detection of small RNA activity

Linda Feldbrugge, BIDMC/Harvard Univsersity
Characterization of circulating microvesicle-associated CD39 family ecto-nucleotidases in human plasma

Brandan Cook, Washington State University
Efficient exosome capture strategy for disease biomarker discovery using engineered peptides

Open/Poster Viewing

Session VII
Chair: Antonio de Maio, UCSD

Michael Graner, University of Colorado
Transcriptional regulator proteins in EVs: the potential to mediate downstream pathways

Dolores Di Vizio, Cedars-Sinai Medical Center
Extracellular Vesicles in Cancer: The Emerging Role of Large Oncosomes

Quan Lu, Harvard School of Public Health
Non-canonical Notch Signaling via Secreted ARMMs

Leonora Balaj, MGH/Harvard University
Profiling of serum and plasma derived extracellular vesicles miRNA and mRNA.

Poster Session B – food and open bar

October 13, 2014
Session VIII
Chair: Stephen Gould, Johns Hopkins University

Paul Robbins, Scripps Institute
Transplantation of young adult stem cells prolongs lifespan and healthspan in a mouse model of accelerated aging: a role for microvesicles

Johan Skog, Exosome Diagnostics
Exosomes: From tissue to liquid biopsy

Maria Zanone, University of Turin, Italy
Human mesenchymal stem cell-derived microvesicles modulate T cell response to islet antigen glutamic acid decarboxylase in patients with type 1 diabetes

Masato Mitsuhashi, Hitachi CRI
Luminal Extracellular Vesicles (EVs) in patients with Inflammatory Bowel Disease (IBD)

Young Chan Choi, Hanyang University, South Korea (60) Extracellular Vesicles Secreted During Human Myoblast Differentiation Regulate Myotube Formation of Human Adipose-Derived Stem Cells

Lilian Cruz, University of Sao Paulo, Brazil
Extracellular vesicles biogenesis during neuronal differentiation of mouse embryonic stem cells: from ultrastructural analysis to cargo protein identification

Go-Woon Kim, KIST, South Korea
Wnt cutter (Wnc) is required for cleavage of exosomal Wingless(Wg) and enhances Wg signaling

Sang-Ho Kwon, UC San Francisco
Intercellular exosome transfer in HGF- induced invasive epithelial growth

Elisa Lázaro-Ibáñez, University of Helsinki, Finland
Presence of Oncogenic Genomic DNA Sequences in Extracellular Vesicles

Shannon Holliday, University of Florida
Osteoclast derived exosomes regulate calcitriol-stimulated osteoclast differentiation in vitro

Michael Dinkins, Georgia Regents University
Exosome reduction in vivo is associated with lower amyloid plaque load in the 5XFAD mouse model of Alzheimer’s disease